Fluorescent In Situ Hybridization Probe Design

Curr opin biotechnol.

Fluorescent in situ hybridization probe design. Fluorescence in situ hybridization fish is a molecular cytogenetic technique that uses fluorescent probes that bind to only those parts of a nucleic acid sequence with a high degree of sequence complementarity it was developed by biomedical researchers in the early 1980s to detect and localize the presence or absence of specific dna sequences on chromosomes. Wagner m haider s. Fluorescence in situ hybridization fish is a technique that uses fluorescent probes which bind to special sites of the chromosome with a high degree of sequence complementarity to the probes. Where the insert may contain a specific gene or originate from a specific chromosomal.

New trends in fluorescence in situ hybridization for identification and functional analyses of microbes. The dna probe and the target dna to which the probe will be hybridized. The dna probe typically comes from cloned sources such as plasmids cosmids pacs yacs or bacs. Fluorescence in situ hybridization fish of genes and mrna is most often based on polynucleotide probes.

The fluorescent probes are nucleic acid labeled with fluorescent groups and can bind to specific dna rna sequences. Multiplex fluorescence in situ hybridization fish enables you to assay multiple targets and visualize colocalized signals in a single specimen. Due to the high allele diversity of genes a single probe is not sufficient to detect all alleles of a gene.